Comparison
of expression levels using Quantitative -RT-PCR of genes differentially
regulated
in
normal*, preimmortal**, and immortal*** cells after 5AZA-dC-induced DNA
demethylation
(increases in gene expression indicated
in red)
|
Gene name
|
1502 vs.
1502 5aza*
|
041LP vs
041LP 5aza**
|
041HP vs
041HP5aza***
|
|
OAS1
|
2
|
5
|
4379
|
|
IRF7
|
1.5
|
1.7
|
2003
|
|
IFN-inducible 56 kDa protein
|
1.2
|
1.2
|
1807
|
|
IFN-inducible protein p27
|
84
|
16
|
1320
|
|
AIM2
|
2.6
|
22
|
1094
|
|
MGSA
|
3.2
|
1.1
|
839
|
|
IFN-inducible protein p78
|
1.8
|
3
|
478
|
|
IFITM1
|
1.8
|
1.25
|
278
|
|
OAS2
|
1.3
|
1.5
|
231
|
|
IFN-induced 17-kDa/15-kDa
|
1.8
|
1.4
|
228
|
|
CIG49
|
1.1
|
1.1
|
204
|
|
STAT1
|
1.8
|
4
|
158
|
|
MIF
|
1.7
|
2
|
128
|
|
NDN
|
1.4
|
1.6
|
10
|
|
IP30
|
5
|
1.6
|
7
|
* Fold change of gene expression level
in a non-immortal fibroblast cell line 1502 MDAH041 before and after treatment
with 5AZA-dC.
** Fold change of gene expression level
in a non-immortal fibroblast cell line MDAH041 low passage before and after
treatment with 5AZA-dC.
*** Fold change of gene expression level
in an immortal fibroblast cell line (MDAH041, high passage) before and
after treatment with 5AZA-dC.
