Genes Differentially Regulated after Immortalization and 5-AZA-Deoxycytidine Treatment *
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Accession #
Gene name
IMMORT
5AZA
Gene Selection Method
L19686 
Macrophage migration inhibitory factor  (MIF)
-278.0
42.1
N/ANOVA
X54489 
Melanoma growth stimulatory activity (MGSA) (GRO-1)
-146.3
64.6
N
 AI017574
Cysteine-rich heart protein
-85.0
8.8
N
M33882 
Interferon-induced p78 Mx1
-75.7
162
N/ANOVA**
U66711 
Ly-6-related protein (9804) gene (responsive to IFNs)
-73.7
34.1
N/ANOVA
X82494
Fibulin-2
-70.1
21.8
N/ANOVA
 AF054825
VAMP5 (vesicle-associated membrane protein 5)
-69.1
9.1
N
 AL049946
Adlican
-60.2
17.6
N/ANOVA**
M55153 
Transglutaminase (TGase)
-50.9
144.6
N
AF037335 
Carbonic anhydrase precursor (CA 12)
-47.4
8.9
N
 L24564
Rad (Ras associated with diabetes)
-35.9
19.7
N/ANOVA**
 AA631972
NK4 protein (natural killer cell transcript 4)
-35.0
20.2
N/ANOVA
 U20982
Insulin-like growth factor binding protein-4
-33.3
3.8
N**
 AF039103
Tat-interacting protein TIP30
-30.3
8.9
N
 J03909
Gamma-interferon-inducible protein (IP-30)
-27.8
31.2
N/ANOVA
 AF053944
Aortic carboxypeptidase-like protein
-27.4
12.3
N/ANOVA
 AL080059
cDNA DKFZp564H142 
-23.4
9.5
N
U88964 
HEM45 (interferon-stimulated gene 20-kd; ISG20)
-21.7
50.2
N/ANOVA
U03688 
Dioxin-inducible cytochrome P450 (CYP1B1)
-20.6
6.9
N
 U59185
Putative monocarboxylate transporter
-19.5
6.3
N
AB029000 
KIAA1077 protein Sulfatase FP
-18.6
10.9
N
U45878 
Inhibitor of apoptosis protein 1
-18.4
13.1
N/ANOVA
M28130 
Interleukin 8
-15.5
92.7
N/ANOVA
 X04371
(2’-5’) oligoadenylate synthetase E OAS-1
-15.4
76.5
N/ANOVA**
X02419 
uPA gene (urokinase-plasminogen activator gene)
-14.6
4.4
N
 M13509
Skin collagenase MMP1
-14.4
4.8
N
 AF026941
CIG5 (cytomegalovirus induces interferon-responsive)
-13.9
66.6
N
 AB025254
PCTAIRE 2 (pctaire protein kinase)
-13.7
19.3
N/ANOVA
X67325 
Interferon-stimulated gene p27 mRNA
-13.0
482.0
N
 M36820
Cytokine (GRO-beta; GRO-2)
-12.9
29.6
N/ANOVA
 AF026939
CIG49 (cytomegalovirus induces interferon-responsive)
-12.8
70.2
N/ANOVA
M90657 
Tumor antigen (L6)
-12.6
17.5
N/ANOVA**
 AF060228
Retinoic acid receptor responder 3
-12.2
7.1
N/ANOVA
J04164 
Interferon-inducible protein 9-27 (IFITM1)
-11.8
8.8
N
 AI885852
Similar to gb:L19779 HISTONE H2A.1
-7.3
10.3
N/ANOVA**
 M36821
Cytokine (GRO-gamma)
-11.1
56.8
N
 AL050162
TESTIN 3  testis derived transcript (3 LIM domains)
-10.7
8.1
N
 U77643
K12 protein precursor (SECTM1)
-10.7
19.6
N/ANOVA
 D28137
BST-2 (bone marrow stroma cell surface gene)
-9.9
38.7
N/ANOVA
 M17017
Beta-thromboglobulin-like protein
-9.7
17.5
N/ANOVA
 AC004142
BAC clone RG118D07 from 7q31
-9.4
5.5
N
 M24283
Major group rhinovirus receptor (HRV)
-9.3
28.9
N
 AL022723
HLA-F gene for major histocompatibility complex classI F
-8.9
29.4
N/ANOVA**
U15932 
DUSP5 (dual specificity MAP kinase-phosphatase)
-8.9
12.6
N/ANOVA
 M24594
Interferon-inducible 56 Kd protein
-8.6
36.6
N/ANOVA**
AB020315
Dickkopf-1 (hdkk-1)
-8.3
14.4
ANOVA
J02931 
Placental tissue factor (two forms)
-8.0
8.7
N
 X86163
B2-bradykinin receptor 3
-7.9
3.8
N
M13755 
Interferon-induced 17-kDa/15-kDa protein 
-7.6
17.0
N/ANOVA**
 L20817
Tyrosine protein kinase (CAK) gene
-7.5
5.7
N
 AJ225089
(2’-5’) oligoadenylate synthetase 59 kDa OAS-L
-7.4
40.0
N/ANOVA
 AF085692
Multidrug resistance-associated protein 3B
-7.3
13.9
N
 M26326
Keratin 18
-6.9
13.5
N
 M22489
Bone morphogenetic protein 2A
-6.8
9.9
N
U37518 
TNF-related apoptosis inducing ligand TRAIL 
-6.7
42.2
N
M92357 
B94 protein (tumor necrosis factor-alpha-inducible)
-6.7
5.5
N
X07523 
Complement factor H
-6.6
6.8
N
 AB018287
KIAA0744 protein
-6.5
8.6
N
 U53831
Interferon regulatory factor 7
-6.3
17.5
N/ANOVA
 X55110
Neurite outgrowth-promoting protein
-6.2
7.1
N
 AL039458
Integral membrane glycoprotein LIG-1 (TM4SF1)
-6.1
5.2
N
AL021977
Transcription Factor MAFF
-6.1
8.1
ANOVA
 M65292
Factor H homologue
-5.8
7.5
N
 D29992
Placental protein 5 (PP5)
-5.7
29.3
N
 AF070533
Optineurin-like protein
-5.7
4.2
N**
AF052135 
Associated molecule with the SH3 domain of STAM
-5.6
7.6
ANOVA
D28915 
Microtubular protein p44
-5.5
17.8
N/ANOVA
M62402 
Insulin-like growth factor binding protein 6
-5.5
5.9
N/ANOVA
 AF024714
Interferon-inducible protein AIM2 (absent in melanoma)
-5.3
21.6
N
M31165 
Tumor necrosis factor-inducible (TSG-6) 
-5.2
10.3
N
U81607 
Gravin
-5.1
12.2
ANOVA
M30818
Interferon-inducible protein; myxovirus resistance Mx2
-5.0
42
N
 M25915
Complement cytolysis inhibitor (CLI) 
-4.9
5.1
N
 AB013382 
DUSP6 (dual specificity MAP kinase-phosphatase)
-4.7
4.6
N
AB000115 
mRNA expressed in osteoblast
-4.3
32.5
N/ANOVA
 D50919
Tripartite motif-containing protein 14, TRIM14
-4.3
6.2
N
 X58536
HLA class I locus C heavy chain
-4.1
5.7
N
AF010312
Pig 7
-4
9.4
ANOVA
 AI985272
Neuromedin B Precursor
-3.9
5.6
N
 X57985
Genes for histones H2B.1 and H2A
-3.6
4.3
N
 U07919
Aldehyde dehydrogenase 6
-3.5
4.1
N
 AA883502
Ubiquitin-conjugating enzyme E2L 6 (UBE2L6)
-3.4
5.9
N
U22970
Interferon-inducible peptide (6-16)
-3.3
10.2
ANOVA
M87434 
(2’-5’) oligoadenylate synthetase 69/71 kDa  OAS-2
-2.7
19.6
N
 M97935
Transcription factor ISGF-3 (Stat 1)
-1.9
7.6
N**

*Common set of genes downregulated with a fold change >2 in the immortal cells relative to non-immortal cells and upregulated genes in the immortal MDAH041 cells treated with 5AZA-dC vs. untreated.  Negative numbers indicate fold change of downregulated gene expression and positive numbers indicate fold change of upregulated gene expression.  Data shown were obtained from Affymetrix HU95Av2 microarrays.  Gene Selection Method: indicates the selection method that identified the particular change in gene expression; N (N-fold change, N=2); ANOVA (99.99% confidence). Gene downregulation after immortalization indicated in column IMMORT and by demethylation indicated in column 5AZA.
** The fold changes for genes with multiple probe set ID’s were averaged.