Genomics Core

Genomics Core

Mission of the Core

The Genomics Core / Applied Genomics Technology Center (AGTC), provides state-of-the-art genomic technologies and expert consultation services. Core scientists interact with researchers at the design phase of projects and assist in incorporating genomics approaches by generating preliminary data, providing methodology descriptions, analyzing cost, and performing high throughput implementation of technology. A genomics analyst group is available to incorporate seamless data management and analysis to genomic data generated by the AGTC.

Core Services Available

Core services are divided into three categories:

  1. Fully established services
  2. Fee for service with established prices, which may vary depending on sample type, quantity, and reagent availability, (genotyping)
  3. Studies requiring new technology development.

Services include:

  • DNA Isolation
  • DNA Sequencing  
  • Genotyping Services  
  • Digital PCR
  • DNA Methylation Studies  
  • RNA Isolation  
  • Expression Analysis  
  • Genomics Data Management and Analysis  

Instrumentation

  • Qiagen Symphony
  • Qiagen EZ1 Advanced-2
  • Qiagen TissueLyser
  • Applied Biosystems 3730
  • Applied Biosystems QuantStudio 12K Flex Real-Time PCR System with robotic stacker
  • Illumina iScan with Tecan freedom evo robot customized to run Illumina protocols and an Illumina AutoLoader
  • Affymetrix GeneChip Scanner 3000 with Autoloader, two GeneChip Fluidics Station 450, GeneChip Hybridization Oven 640, two Affymetrix workstations
  • Agilent 2100 Bioanalyzer
  • Agilent TapeStation
  • NanoDrop ND-1000 Spectrophotometer
  • Trinean DropSense96 Spectrophotometer
  • Eppendorf epMotion 5075-2 pre-PCR units and a post-PCR unit
  • Covaris S2 (adaptive acoustic focused energy for shearing nucleic acid)
  • Illumina HiSeq 2000 (to be upgraded to a 2500 in late 2012)
  • Primary Server for Illumina Pipeline (Dell R910 with 32 cores, 1TB memory, and 10 TB local storage
  • Backup Server for Illumina Pipeline (Dell R900 with 16 cores, 64 GB memory, and 27 TB storage)
  • Failover and Analysis Server (Dell R900 with 16 cores, 256 GB memory, and 8 TB local storage)
  • Testbed Server Dell R900 with 16 cores, 128 GB memory, and 27 TB storage)
  • Sunfire X4500 with 48 TB storage with a second identical unit at WSU C&IT acting as a mirror
  • Sunfire X4540 with 96 TB storage with a second identical unit at WSU C&IT acting as a mirror
  • Illumina Genome Studio-5 licenses
  • Novoalign License
  • Genomatix

Additional Information

Core Leadership

Susan J. Land, Ph.D.
Director, Genomics Core
sland@med.wayne.edu

Aliccia Bollig-Fischer, Ph.D.
Associate Director, Genomics Core
bollig@karmanos.org

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